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S. Jain , M. D. Kharya , S. Nayak , R. Barik

Abstract

Objective: In the present study, adsorbents and antioxidants incorporated in to callus cultures of Glycyrrhiza glabra to study their potency in checking callus browning. Methods: Activated charcoal was added as an adsorbent at varying concentrations of 100, 500 and 1000 mg/l. Ascorbic acid and cysteine were added as antioxidants each at concentrations of 50, 100 and 150 mg/l grown on Modified MS basal medium supplemented with growth regulators (BA, Kn, IAA, NAA). Result: Browning was effectively controlled by activated charcoal and ascorbic acid while cysteine did not show any inhibition. Discussion: Browning is a major problem affecting tissue culture studies of medicinal plants containing polyphenolic compounds that cause oxidation reactions leading to browning. Hence browning could be controlled by using adsorbents that adsorb the oxidative by products or by using antioxidants that prevent the oxidation reactions.

Keywords

Glycyrrhiza glabra, Antioxidants, Callus Browning, Necrosis

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H. N. Shivaprasad , M. D. Kharya , A. C. Rana

Abstract

Objective: To investigate the antioxidant and adaptogenic activities of Triphala, an Indian Ayurvedic medicinal preparation. Materials and Methods: Antioxidant activity of Triphala was determined by hydroxyl and nitric oxide radical scavenging methods. Adaptogenic activity was studied using swim endurance, anoxic stress tolerance and chronic stress induced behavioral despair test models. Triphala was administered at the dosage levels of 100 to 500 mg/kg b.w.p.o. Results and Discussion: Triphala was found to scavenge hydroxyl and nitric oxide radicals in vitro. The IC₅₀ value for hydroxyl radical scavenging was 40.5 μg/mL and that for nitric oxide radical scavenging was found to be 40 μg/mL, respectively. Oral administration of Triphala formulation significantly improved the stress tolerance by increasing the swim duration (762.28 ± 7.17 minute), anoxic stress tolerance duration (39.11 ± 1.05 minute) and reduced the stress induced increase in the immobility period (61.11 ± 3.42 seconds) in chronic shock induced stress. Conclusion: Triphala has been found to be an excellent scavenger of hydroxyl radicals and nitric oxide radicals, whose excessive formation is implicated in oxidative stress. Triphala is capable of increasing the capacity to tolerate non-specific stress in experimental animals as evident from the restoration of parameters studied during different types of stress models.

Keywords

Triphala, Antioxidant, Adaptogenic

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Mohammad Yasir ¹, Sattwik Das ¹, Neelesh Kumar Mehra ¹, M. D. Kharya ²

Affiliations
1 Department of Pharmacy, Malhotra College, Bhopal-(M.P), IN
2 Department of Pharmaceutical Sciences, H. S. Gour University, Sagar (M.P), IN

Abstract

Over the past decades, herbal medicine has become a thing of global significance with medicinal and economic implications .Wide spread use of herbs throughout the global has raised serious concerns over this quality, safety and efficacy .Thus exact scientific assessment has become a precondition for acceptance of herbal health claims. Pleione bulbocodioides (Orchid) is a tree, native to France, they are well adapted to cold temperatures and even frost belongs to the family Orchidaceae and is widely used in chinies herbal medicine. There are approx twenty species of pleione. The plant is used in traditional medicine for the treatment of various ailments such as antimicrobial,anticancer and antiallergic. From tubers of Pleione bulbocodioides number of phytoconstituents are isolated which has significant pharmacological activity.

Keywords

Pleione bulbocodioide, Stilbenoids, Bichroman.

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